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Antigenic Specificity | Vimentin Protein |
Clone | polyclonal |
Host Species | Chicken |
Reactive Species | human, mouse, and rat |
Isotype | IgY |
Format | antigen affinity purified |
Size | 1000 µL |
Concentration | 300 µg/mL |
Applications | IHC, ICC, WB |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the vimentin gene product, but are shared between the human (NP_003371, NCBI) and mouse (NP_035831, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter sterilized. RRID: AB_2313565 |
Immunogen | Synthetic peptides |
Other Names | Vimentin |
Gene, Accession # | VIM, UniProt: P08670 |
Catalog # | VIM |
Price | $455 |
Order / More Info | Vimentin Protein Antibody from AVES LABS |
Product Specific References | n/a |