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Antigenic Specificity | Nestin |
Clone | polyclonal |
Host Species | Chicken |
Reactive Species | mouse and rat |
Isotype | IgY |
Format | antigen affinity purified |
Size | 100 µL |
Concentration | 300 µg/mL |
Applications | IHC, ICC, WB |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. RRID: AB_2314882 |
Immunogen | Synthetic peptides |
Other Names | Nestin |
Gene, Accession # | NES Nbla00170, UniProt: Q6P5H2 |
Catalog # | NES-0020 |
Price | $135 |
Order / More Info | Nestin Antibody from AVES LABS |
Product Specific References | n/a |