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Antigenic Specificity | Amyloid Precursor Protein (APP), Peptide #3, #4, #5 |
Clone | polyclonal |
Host Species | Chicken |
Reactive Species | human, mouse, and rat |
Isotype | IgY |
Format | antigen affinity purified |
Size | 1000 µL |
Concentration | 300 µg/mL |
Applications | IHC, ICC |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Chickens were immunized with keyhole limpet hemocyanin (KLH) conjugates of three different synthetic peptides (named #3, #4 and #5). Peptide #3 is QHF QEK VES LEQ EAA NER QQ (which corresponds to residues #433-452 of the human sequence NP_000475.1); peptide #4 is EQK DRQ HTL KHF EHV RMV DPK K (residues #501-522); peptide #5 is EEI QDE VDE LLQ KEQ NYS DD (residues #556-575). It should be noted that these three peptide sequences are 100% identical with the mouse (P12023.3) and rat (P08592.1) gene products. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified against each of the three cognate peptides using agarose affinity matrices. The eluate concentrations were then adjusted to 300 µg/mL, mixed in equimolar concentrations (100 µg/mL is the final concentration of each antibody), and the preparation was filter-sterilized. RRID: AB_2797315 |
Immunogen | Synthetic peptides (QHF QEK VES LEQ EAA NER AA {residues #433-452}, EQK DRQ HTL KHF EHV RMV DPK K {residues #501-522}, EEI QDE VDE LLQ KEQ NYS DD, residues #556-575}) from the human APP gene product |
Other Names | APP, Amyloid-beta A4 protein, Amyloid beta, beta Amyloid |
Gene, Accession # | APP A4 AD1, UniProt: P05067 |
Catalog # | APP345 |
Price | $455 |
Order / More Info | Amyloid Precursor Protein (APP), Peptide #3, #4, #5 Antibody from AVES LABS |
Product Specific References | n/a |