AKT Phospho (Thr308) Antibody from BIOLEGEND

AKT (also known as protein kinase B alpha) is a 56 kDa serine/threonine-specific kinase containing a pleckstrin domain. AKT plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon cell activation. It is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2. Activated AKT phosphorylates a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3beta, Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). AKT promotes cell survival by inhibiting apoptosis through phosphorylation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. AKT also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, AKT phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.