Edit |   |
---|---|
Antigenic Specificity | BirA |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | serum |
Size | 100 ul |
Concentration | 70 mg/ml |
Applications | ELISA, WB |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BLT of BirA). Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown). |
Immunogen | Anti-Biotin Ligase Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic avi-tag peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine). |
Other Names | rabbit Biotin Ligase Epitope Tag, rabbit Avi-tag, biotin ligase, biotin operon repressor protein, biotin holoenzyme synthetase, biotin-[acetyl-CoA carboxylase] synthetase |
Gene, Accession # | n/a |
Catalog # | orb750518 |
Price | $665 |
Order / More Info | BirA Antibody from BIORBYT LTD. |
Product Specific References | n/a |
U.S. OFFICE
Biorbyt LLC
1100 Corporate Square Drive
Helix Center, Suite 221
St Louis, MO 63132
Email: info@biorbyt.com
Phone: +1 (415) 906-5211
Fax: +1 (415) 651-8558