C-FOS Antibody from IMMUNOSTAR INC.
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| Edit | |
|---|---|
| Antigenic Specificity | C-FOS |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | mouse, rat |
| Isotype | n/a |
| Format | serum |
| Size | 100 µl |
| Concentration | n/a |
| Applications | Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | The C-FOS Antibody was raised to synthetic peptide sequence corresponding to human c-fos (4-17) coupled to bovine thyroglobulin with glutaraldehyde. For induction of c-fos protein activity rats were injected with 1.0 ml of 1.5 M NaCl per 100 grams of body weight. Negative control rats were injected with the same volume of normal saline. The ImmunoStar c-fos antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat paraventricular nucleus and supraoptic nucleus using indirect immunofluorescent and biotin/avidin-HRP techniques. No labeling was seen in negative control rats. Recommended primary dilutions for these methods are 1/200-1/400 in PBS/0.3% Triton X-100 - FITC Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Specificity of the antiserum was demonstrated by blockage of staining in experimental rats by omission of c-fos antibody or by substitution of antibody pre-incubated with synthetic peptide or the conjugate. Immunoblot analysis of mediobasal hypothalamus showed a single band of approximately 55-60 kD. |
| Immunogen | Synthetic human c-fos (4-17) |
| Other Names | n/a |
| Gene, Accession # | n/a |
| Catalog # | 26209 |
| Price | $425 |
| Order / More Info | C-FOS Antibody from IMMUNOSTAR INC. |
| Product Specific References | PubMed: 23262236, 23085313, 21933288, 20214438, 16533665, 15219664, 12676287, 12353219, 10362318 |
IMMUNOSTAR INC.
IMMUNOSTAR INC.
P.O. Box 488
Hudson WI 54016-0488
P: 715-386-8407
P: 866-386-3500
F: 866-386-4500
info@immunostar.com
http://www.immunostar.com
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