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Antigenic Specificity | Ly-49E/F, Mouse |
Clone | REAL331 |
Host Species | Recombinant Human |
Reactive Species | mouse |
Isotype | n/a |
Format | phycoerythrin (PE) conjugate |
Size | 30 µg in 200 µL |
Concentration | 1:50 |
Applications | Flow cytometry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Ly-49E/F Antibody, anti-mouse, PE, REAlease®. Clone REAL331 is an antibody fragment derived from the full Ly-49E/F antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL331 recognizes the mouse killer cell lectin-like receptor 5 and 6 (Ly-49E and Ly-49F) antigens, which are type II transmembrane natural killer cell receptors belonging to the Ly-49 family. Members of the Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands, and include activating and inhibitory receptor molecules. Ly-49E is expressed abundantly on fetal or immature natural killer (NK) cells and on certain γδ T cells, but at barely detectable frequencies on adult splenic NK cells. Different from other Ly-49 receptors, Ly-49E contains an immunoreceptor tyrosine-based inhibitory motif domain, a charged transmembrane residue and it is present in all mouse strains. Its failure to bind to a variety of soluble or cell bound MHC class I molecules indicates that its natural ligand is not an endogenous class I molecule. Interleukin 2 (IL-2) and IL-15 selectively induce the de novo expression of Ly-49E on the majority of mature NK cells. By contrast, IL-4 blocks this IL-2 induced acquisition of Ly-49E. Ly-49F is expressed on subsets of NK cells, NK-T cells, and on a subpopulation of memory T cells in C57BL/6 mice, but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice. IL-21 selectively down-regulates Ly-49F on mature NK cells. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes. |
Immunogen | n/a |
Other Names | Klra5, Ly49e, Ly49F, Klra6 |
Gene, Accession # | n/a |
Catalog # | 130-118-530 |
Price | $109 |
Order / More Info | Ly-49E/F, Mouse Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | n/a |