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Antigenic Specificity | CD125 (IL-5Rα), Mouse |
Clone | REA343 |
Host Species | Recombinant Human |
Reactive Species | mouse |
Isotype | IgG1 |
Format | allophycocyanin (APC) conjugate |
Size | 30 µg in 200 µL |
Concentration | 1:50 |
Applications | Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | CD125 (IL-5Rα) Antibody, anti-mouse, APC, REAfinity™. Clone REA343 recognizes the mouse CD125 antigen, a single-pass type I membrane protein, also known as interleukin-5 receptor subunit α (IL-5Rα). The IL-5 receptor is a heterodimer composed of a ligand specific α subunit and a signal transducing β subunit which is shared by the receptors for IL-3, GM-CSF, and IL-5. CD125 is expressed on eosinophils, basophils, and B cells. IL-5 is a conserved hematopoietic cytokine which is mainly produced by T?2 cells after stimulation with antigens, and by mast cells upon stimulation with an allergen/IgE complex or a calcium ionophore. The overexpression of IL-5 in vivo significantly increases the numbers of eosinophils and B cells, while mice lacking the functional gene for IL-5 display various developmental and functional impairments in the B cell and eosinophil lineages. | Additional information: Clone REA343 displays negligible binding to Fc receptors. | |
Immunogen | n/a |
Other Names | CDw125, Il5r, IL-5RA |
Gene, Accession # | Gene ID: 16192 |
Catalog # | 130-118-689 |
Price | $93 |
Order / More Info | CD125 (IL-5Rα), Mouse Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Takaki, S. et al. (1990) Molecular cloning and expression of the murine interleukin-5 receptor. EMBO J. 9 (13): 4367-4374 . | Mao, H. et al. (2006) Effect of interleukin-5 receptor-alpha short hairpin RNA-expressing vector on bone marrow eosinophilopoiesis in asthmatic mice. Adv. Ther. 23 (6): 938-956. | Kusano, S. et al. (2012) Structural basis of interleukin-5 dimer recognition by its α receptor. Protein Sci. 21 (6): 850-864. |