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Antigenic Specificity | IL-5, Human |
Clone | JES1-39D10 |
Host Species | Rat |
Reactive Species | human, nonhuman primate |
Isotype | IgG2aκ |
Format | Vio R667 conjugate |
Size | 30 tests in 300 µL |
Concentration | 1:10 |
Applications | Intracellular flow cytometry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | IL-5 Antibody, anti-human, Vio® R667. Interleukin 5 (IL-5) is pre-dominantly secreted by CD4+ T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells. | The Anti-IL-5 antibody has been designed for intracellular staining of IL-5-producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5-producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5-producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS® Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4+ T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids. |
Immunogen | n/a |
Other Names | EDF, TRF |
Gene, Accession # | Gene ID: 3567 |
Catalog # | 130-111-931 |
Price | $189 |
Order / More Info | IL-5, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | n/a |