Edit |   |
---|---|
Antigenic Specificity | IFN-γ, Human |
Clone | 45-15 |
Host Species | Mouse |
Reactive Species | human, nonhuman primate |
Isotype | IgG1κ |
Format | fluorescein (FITC) conjugate |
Size | 100 tests in 200 µL |
Concentration | 1:50 |
Applications | Intracellular flow cytometry |
Reviews / Ratings | |
Description | IFN-γ Antibody, anti-human, FITC. Interferon gamma (IFN-γ) is produced by cells that are involved in inflammatory immune responses. IFN-γ is predominantly released by memory and effector CD4+ and CD8+ T cells as well as by NK cells upon activation. Quantitative analysis of IFN-γ-producing cells can provide important information on the course of immune responses. Anti-IFN?γ antibodies are designed for intracellular staining of IFN?γ-producing cells. Cells can be stimulated for IFN-γ-production, e.g. by polyclonal stimulation with mitogens. For induction of IFN?γ-production by antigen-specific T cells, cells are restimulated with respective antigen. IFN?γ can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IFN?γ-producing cells can be stained intracellularly with Anti-IFN?γ antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IFN-γ-producing cells. |
Immunogen | n/a |
Other Names | IFNg, IFG, IFI |
Gene, Accession # | Gene ID: 3458 |
Catalog # | 130-113-492 |
Price | $275 |
Order / More Info | IFN-γ, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | Chaput, N. et al. (2013) Phase I clinical trial combining imatinib mesylate and IL-2: HLA-DR+ NK cell levels correlate with disease outcome. Oncoimmunology 2 (2): e23080. |