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Antigenic Specificity | TNF-α, Human |
Clone | cA2 |
Host Species | Human |
Reactive Species | human |
Isotype | IgG1 |
Format | phycoerythrin (PE) conjugate |
Size | 30 tests in 60 µL |
Concentration | 1:50 |
Applications | Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | TNF-α Antibody, anti-human, PE. Tumor necrosis factor alpha (TNF-α) is produced by cells that are involved in inflammatory immune responses. TNF-α is secreted by activated CD4+ T cells, monocytes, macrophages, NK cells, and neutrophils. | Anti-TNF-α antibodies have been designed for intracellular staining of TNF-α-producing cells. Cells can be stimulated for TNF-α production, for example, by polyclonal stimulation with mitogens. For induction of TNF-α production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-α can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-α-producing cells can be stained intracellularly with Anti-TNF-α antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-α-producing cells. |
Immunogen | n/a |
Other Names | TNF, DIF, TNFSF2 |
Gene, Accession # | Gene ID: 7124 |
Catalog # | 130-120-627 |
Price | $119 |
Order / More Info | TNF-α, Human Antibody from MILTENYI BIOTEC B.V. & Co. KG |
Product Specific References | n/a |