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Antigenic Specificity | FLAG-tag |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | immunogen affinity purified |
Size | 0.03 mL, 0.1 mL, 0.2 mL |
Concentration | 1 mg/ml |
Applications | Western Blot (WB), ELISA (EIA) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Specificity: FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells. The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistr |
Immunogen | Immunogen: DDDDK synthetic peptide conjugated to KLH |
Other Names | [Rabbit FLAG-tag; DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope] |
Gene, Accession # | [FLAG] |
Catalog # | MBS9700851 |
Price | $130, $220, $325 |
Order / More Info | FLAG-tag Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |