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Antigenic Specificity | Unphosphorylated N-Cadherin (Tyr-820) |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, rat, mouse |
Isotype | n/a |
Format | affinity purified |
Size | 0.1 mL |
Concentration | n/a |
Applications | ELISA, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antibody was cross-adsorbed to phospho-Ncadherin (Tyr-820) peptide before affinity purification using unphosphorylated N-cadherin (Tyr-820) peptide. The purified antibody detects a strong band at 130 kDa* in western blots of serum-starved human endothelial cells, but is decreased in pervanadate treated cells. This reactivity in western blots is blocked specifically by unphosphorylated N-cadherin (Tyr-820) peptide. Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering |
Immunogen | Immunogen: Unphosphorylated N-Cadherin (Tyr-820) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 inhuman N-cadherin. This sequence is conserved in rat and mouse Ncadherin, and has three amino acid differences from the conserved region in R-cadherin. |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | MBS472063 |
Price | $385 |
Order / More Info | Unphosphorylated N-Cadherin (Tyr-820) Antibody from MYBIOSOURCE INC. |
Product Specific References | • Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463• Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264.• Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261. |