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Antigenic Specificity | Mouse TNF alpha |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | mouse |
Isotype | n/a |
Format | unconjugated |
Size | 0.1 mg |
Concentration | 1ug/ul |
Applications | Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Radioimmunoassay (RIA) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antibody has been heated to 56 degree C for 30 minutes. The antibody is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFb (lymphotoxin). This antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. |
Immunogen | Immunogen: This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli. |
Other Names | [Mouse TNF alpha] |
Gene, Accession # | [TNF alpha] |
Catalog # | MBS4156825 |
Price | $540 |
Order / More Info | Mouse TNF alpha Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |