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Antigenic Specificity | DYKDDDDK TAG |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | unconjugated |
Size | 0.1 mg |
Concentration | IgG concentration 0.5 mg/ml |
Applications | ELISA (EIA), Western Blot (WB) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Rabbit anti DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays. In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody has been shown not to cross-react with any endogenous proteins. |
Immunogen | Immunogen: Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end. Buffer Solution: Phosphate buffered saline Antiserum Preparation: Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. Target Species: Synthetic Peptide |
Other Names | n/a |
Gene, Accession # | [DYKDDDDK] |
Catalog # | MBS221366 |
Price | $545 |
Order / More Info | DYKDDDDK TAG Antibody from MYBIOSOURCE INC. |
Product Specific References | n/a |