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Antigenic Specificity | Mitotic cells |
Clone | [8B3G] |
Host Species | Mouse |
Reactive Species | human, zebrafish |
Isotype | IgM |
Format | unconjugated |
Size | 0.1 mg |
Concentration | n/a |
Applications | Flow Cytometry (FC), Immunocytochemistry (IHC) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Specificity: 8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorpoRation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle. The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation |
Immunogen | Source Note: 8B3G is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a total cell lysate of the Human bladder carcinoma cell line T24. |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | MBS570109 |
Price | $415 |
Order / More Info | Mitotic cells Antibody from MYBIOSOURCE INC. |
Product Specific References | 1. No references available |