Edit |   |
---|---|
Antigenic Specificity | Akt (N-terminal Region) |
Clone | monoclonal |
Host Species | Mouse |
Reactive Species | human, rat, mouse |
Isotype | IgG1 |
Format | Protein A purified |
Size | 0.1 mL |
Concentration | n/a |
Applications | ELISA, Western Blot, Immunocytochemistry |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of A431 cell lysate. Similar results were seen in various other human, rat, and mouse lysates. Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphoryla |
Immunogen | Immunogen: Clone M101 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Akt1. This sequence ishighly conserved in human and mouse Akt, and may recognize Akt2 and Akt3. |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | MBS470001 |
Price | $325 |
Order / More Info | Akt (N-terminal Region) Antibody from MYBIOSOURCE INC. |
Product Specific References | • Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.• Marte, B. & Downward. J. (1997) TIBS. 22:355-358.• Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.• Smith, M.A. et al. (2007) Am. J. Phys. Cell. Phys. 293:1947. (WB: mouse C2C12)• Moylan, J.S. et al. (2008) Am. J. Phys. Cell. Phys. 295:986. (WB: mouse C2C12) |