AKT1 Antibody Combo Pack Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Antigenic Specificity | AKT1 Antibody Combo Pack |
| Clone | 14E5.A2.B2.H9 |
| Host Species | Mouse |
| Reactive Species | human |
| Isotype | IgG2a kappa |
| Format | Protein A purified |
| Size | 1 Pair |
| Concentration | 1.0 mg/ml |
| Applications | ELISA, Flow Cytometry, Immunohistochemistry, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | This AKT1 primary and secondary antibody pair has been carefully chosen to give best results. Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. This antibody pair is sufficient for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2). This AKT1 Antibody Combo Pack contains: Mouse Anti-AKT1 and Rabbit Anti-Mouse IgG 1, 2a, 2b, 3 HRP Antibody. AKT1 Antibody detects AKT1 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT1 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. |
| Immunogen | Anti-AKT1 Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to internal residues of human AKT1 protein. Anti-Mouse IgG HRP secondary antibody was produced by repeated immunizations in rabbit with Mouse IgG whole molecule. |
| Other Names | AKT 1 antibody pair, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, primary and secondary antibody pair, Primary+Secondary pair, matched antibody pair |
| Gene, Accession # | AKT1, Gene ID: 207, NCBI: NP_001014431.1, UniProt: P31749 |
| Catalog # | K-I51 |
| Price | $159 |
| Order / More Info | AKT1 Antibody Combo Pack Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | n/a |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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