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Antigenic Specificity | Fluorescein |
Clone | polyclonal |
Host Species | Goat |
Reactive Species | n/a |
Isotype | n/a |
Format | DyLight™ 549 conjugate |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | FLISA, IF Microscopy, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Fluorescein Antibody Dylight™ 549 Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight™ antibody and reagent conjugates are specifically designed for protein detection methods that covers much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight™conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging. |
Immunogen | Fluorescein conjugated to Goat IgG |
Other Names | goat Anti-FITC antibody DyLight™ 549 conjugation, DyLight™ 549 conjugated goat anti-fluorescein antibody, DyLight™549 conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody DyLight™ 549 conjugation |
Gene, Accession # | n/a |
Catalog # | 600-142-096 |
Price | $445 |
Order / More Info | Fluorescein Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |