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Antigenic Specificity | Luciferase |
Clone | polyclonal |
Host Species | Goat |
Reactive Species | n/a |
Isotype | n/a |
Format | biotin conjugate |
Size | 25 µL |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-Luciferase Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase. Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others. |
Immunogen | Luciferase [Photinus pyralis (Firefly)] |
Other Names | goat anti-Luciferase Antibody biotin Conjugation, Biotin Conjugated goat anti-Luciferase Antibody, Luciferase Biotin, Anti-Luciferase BAC Antibody |
Gene, Accession # | n/a |
Catalog # | 200-106-150S |
Price | $125 |
Order / More Info | Luciferase Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |