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Antigenic Specificity | NOTCH 2 |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human |
Isotype | n/a |
Format | serum |
Size | 200 µL |
Concentration | 75 mg/mL |
Applications | ELISA, Immunohistochemistry, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities. Anti Notch 2 Antibody recognizes Notch 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
Immunogen | This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling. |
Other Names | rabbit anti-Notch 2 antibody, AGS 2 antibody, AGS2 antibody, hN2 antibody, N2 antibody, Neurogenic locus notch homolog protein 2 antibody, Notch 2 intracellular domain antibody, Notch homolog 2 antibody |
Gene, Accession # | NOTCH2, Gene ID: 4853, NCBI: 24041035, UniProt: Q04721 |
Catalog # | 100-401-406 |
Price | $475 |
Order / More Info | NOTCH 2 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 18418349 // 19433762 // 19828677 |