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Antigenic Specificity | Biotin Ligase Epitope Tag |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | serum |
Size | 100 µL |
Concentration | 70 mg/ml |
Applications | ELISA, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BLT of BirA). Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown). The peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E. coli. |
Immunogen | Anti-Biotin Ligase Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic avi-tag peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine). |
Other Names | rabbit anti-Biotin Ligase Epitope Tag Antibody, rabbit anti-Avi-tag antibody, biotin ligase, biotin operon repressor protein, biotin holoenzyme synthetase, biotin-[acetyl-CoA carboxylase] synthetase |
Gene, Accession # | BirA |
Catalog # | 100-401-B21 |
Price | $399 |
Order / More Info | Biotin Ligase Epitope Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 31495665 |