Mouse Ig Antibody from ROCKLAND IMMUNOCHEMICALS INC.
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| Antigenic Specificity | Mouse Ig |
| Clone | eB144 |
| Host Species | Rat |
| Reactive Species | mouse |
| Isotype | n/a |
| Format | fluorescein (FITC) conjugate |
| Size | 100 µL |
| Concentration | 1.0 mg/mL |
| Applications | FLISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Fluorescent Mouse TrueBlot® Antibody Fluorescein Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA. Mouse IgG TrueBlot® is a unique fluorescein conjugated Anti-mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Fluorescent Western Blot data with Mouse IgG TrueBlot®, simply substitute the conventional FITC Anti-mouse IgG blotting reagent with Fluorescent Mouse TrueBlot® Antibody Fluorescein and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. |
| Immunogen | n/a |
| Other Names | Anti-Mouse IgG FITC, TrueBlot, FITC TrueBlot ULTRA, Fluorescein TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Fluorescent TrueBlot, Ms TrueBlot |
| Gene, Accession # | n/a |
| Catalog # | 18-0217-32 |
| Price | $259 |
| Order / More Info | Mouse Ig Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 30853435 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
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