Edit |   |
---|---|
Antigenic Specificity | DYKDDDDK (FLAG® tag) |
Clone | 29E4.G7 |
Host Species | Mouse |
Reactive Species | n/a |
Isotype | IgG2a kappa |
Format | DyLight™ 800 conjugate |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, FLISA, Flow Cytometry, IF Microscopy, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Antibody for the detection of FLAG™ recognizes FLAG™ and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. Antibody for the detection of FLAG™ can be used to identify fusion proteins containing the FLAG™ epitope. Antibody for the detection of FLAG™ recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. |
Immunogen | This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. |
Other Names | mouse anti-FLAG™ tag DyLight™800 conjugation, Enterokinase Cleavage Site (ECS), DyLight™800 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
Gene, Accession # | n/a |
Catalog # | 200-345-383 |
Price | $425 |
Order / More Info | DYKDDDDK (FLAG® tag) Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |