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Antigenic Specificity | IL-35 EBI3 |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human |
Isotype | n/a |
Format | IgG fraction, no preservative |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-EBI-3 Antibody / Anti-IL27 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from human sources based on 100% homology with the immunizing sequence. Based on 97% homology, there is a chance of cross-reactivity to EBI3 from chimpanzee. Cross-reactivity with EBI-3 from other sources has not been determined. The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses.The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. |
Immunogen | Anti-EBI-3 Antibody / Anti-IL27 is an IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human EBI3 protein produced in E.coli. |
Other Names | rabbit Anti-EBI3, rabbit anti-EBI-3, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35 |
Gene, Accession # | EBI3, Gene ID: 10148, NCBI: NP_005746.2, UniProt: Q14213 |
Catalog # | 209-401-B66 |
Price | $475 |
Order / More Info | IL-35 EBI3 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |