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Product Name | Custom Polyclonal Antibody Production |
Description | Affinity Biologicals has more than 30 years of experience making, purifying and labeling antibodies, purifying proteins, and developing assays. Antibodies are produced in a licensed research facility. Custom polyclonal antibody production in sheep or goats Standard package for custom polyclonal antibody production consists of a 110 day immunization schedule. All animal costs, maintenance and technical costs are included. Antigen for immunization and testing is provided by the customer. Typical antiserum yield at completion is 700 mL per sheep or 500 mL per goat. Sodium azide is added to antiserum to prevent microbial growth. • Antibody purification Fractionation of IgG from antiserum - our method involves octanoic acid precipitation, dialysis and ammonium sulphate precipitation steps. Please inquire about IgG purification up to 1000 mL antiserum. Affinity purification of IgG on immobilized antigen Method involves affinity chromatography of whole IgG on immobilized antigen with the elution of specific antibodies under non-denaturing conditions. Pricing is dependent on the capacity of the antigen resin and the number of affinity-purification cycles required to obtain the required quantity of affinity-purified antibody (APIgG). • Antibody conjugations: Peroxidase Conjugation (using horseradish peroxidase), for 5 mg IgG Biotin Conjugation (using Sulpho-NHS-LC-LC-Biotin), for 5 mg IgG Fluorescein Conjugation (using FITC), for 5 mg IgG |
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Price | please inquire |
Order / More Info | Custom Polyclonal Antibody Production from AFFINITY BIOLOGICALS INC. |
Product Specific References | n/a |