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Product Name | COVID-19 Neutralizing Antibody Quantitatinve ELISA Assay Kit |
Description | This ELISA kit is designed, developed, and produced for the quantitative measurement of the neutralizing antibodies to SARS-CoV-2 RBD of its spike protein in human serum This assay utilizes the microplate-based enzyme immunoassay technique. Assay calibrators, controls, and human serum samples are added to the microtiter wells of a microplate coated with streptavidin. Simultaneously, horseradish peroxidase (HRP) labeled COVID-19 recombinant spike protein and biotinylated angiotensin converting enzyme-2 (ACE-2) are added to each well. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step; a complex of "Streptavidin---Biotin-ACE2---HRP-COVID-19 recombinant spike protein" is formed. If there is specific COVID-19 neutralizing antibody present in the tested specimen, the formation of the above complex is blocked. A color reaction with a substrate solution in a timed reaction is measured in a spectrophotometric microplate reader. The HRP enzymatic activity of the complex on the wall of the microtiter well is inversely proportional to the amount of the COVID-19 neutralizing antibody level in the tested specimen. Sample types: serum. Range: 0 U/ml - 100 U/ml. Incubation time: 1 hr 5 mins |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | COVID-19 Neutralizing Antibody test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | KTR-1036 |
Price | $825 |
Order / More Info | COVID-19 Neutralizing Antibody Quantitatinve ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |