Edit |   |
---|---|
Product Name | Alternative Complement Pathway, Human, Assay |
Description | The Human Alternative Complement Pathway ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent activity assay based on the sandwich principle with a working time of 1 hour and 45 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and positive control are incubated in microtiter wells coated with LPS. Peroxidase-conjugated antibody will bind a neo-epitope of to the formed TCC complex. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. The Human Alternative Complement Pathway activity of samples, which are run concurrently with the standards, can be evaluated on OD-data using regression analysis on logistically transformed values. Complement activity can be expressed as percentage of the activity of a positive standard serum. |
Size | 1 x 96 det. |
Concentration | n/a |
Applications | RUO |
Other Names | Complement Activity Assay |
Gene, Accession, CAS # | n/a |
Catalog # | HK3012 |
Price | $874 |
Order / More Info | Alternative Complement Pathway, Human, Assay from HYCULT BIOTECH |
Product Specific References | n/a |