Alternative Complement Pathway, Human, Assay from HYCULT BIOTECH

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Product NameAlternative Complement Pathway, Human, Assay
DescriptionThe Human Alternative Complement Pathway ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent activity assay based on the sandwich principle with a working time of 1 hour and 45 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and positive control are incubated in microtiter wells coated with LPS. Peroxidase-conjugated antibody will bind a neo-epitope of to the formed TCC complex. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. The Human Alternative Complement Pathway activity of samples, which are run concurrently with the standards, can be evaluated on OD-data using regression analysis on logistically transformed values. Complement activity can be expressed as percentage of the activity of a positive standard serum.
Size1 x 96 det.
Concentrationn/a
ApplicationsRUO
Other NamesComplement Activity Assay
Gene, Accession, CAS #n/a
Catalog #HK3012
Price$874
Order / More InfoAlternative Complement Pathway, Human, Assay from HYCULT BIOTECH
Product Specific Referencesn/a
HYCULT BIOTECH
HYCULT BIOTECH
HYCULT BIOTECH
Frontstraat 2a
5405 PB Uden THE NETHERLANDS
P: +31 (0)413 251335
F: +31 (0)413 248353

info@hycultbiotech.com

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