Adiponectin ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | Adiponectin ELISA |
| Description | The assay utilizes the two-site ``sandwich'' technique with one monoclonal and one polyclonal antibody that bind to human Adiponectin. Standards, controls and diluted patient samples which are assayed for human Adiponectin are added to wells of microplate coated with a high affine monoclonal anti-human Adiponectin antibody. During the first incubation step, Adiponectin in the samples is bound by the immobilized antibody. Then a peroxidase labeled conjugate is added to each well and the following complex is formed: capture antibody - human Adiponectin - Peroxidase conjugate. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the Adiponectin concentration of sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from standard. Adiponectin present in the patient samples, is determined directly from this curve. Range: 1.4-88 ng/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Adiponectin |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 6250 |
| Price | please inquire |
| Order / More Info | Adiponectin ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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