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Product Name | Anti ox-LDL ELISA |
Description | This ELISA is designed for the quantitative determination of ox-LDL antibodies. This assay is a sandwich ELISA for the direct measurement of ox-LDL antibodies in human EDTA plasma and serum. Standards, controls and samples containing human anti ox-LDL antibodies are added into the wells of a microplate coated with malondialdehyde-modified low-density lipoprotein (MDA-LDL, ox-LDL). During the first incubation period, ox-LDL immobilised on the wall of the microtiter wells, captures the antibodies in the samples. After washing away the unbound components, a peroxidase-labeled conjugate is added into each microtiter well. Tetramethylbenzidine is used as a peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The intensity of the yellow colour is directly proportional to the ox-LDL antibody concentration of the sample. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. The ox-LDL antibody concentration in the samples is determined directly from this curve. Range: 30-1000 mU/mL |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Anti ox-LDL |
Gene, Accession, CAS # | n/a |
Catalog # | K 7809 |
Price | please inquire |
Order / More Info | Anti ox-LDL ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |