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Product Name | CRP ELISA |
Description | This ELISA is designed for the quantitative determination of CRP in serum, plasma, urine and stool samples. The wells of the microtiter plate are coated with polyclonal antibodies directed against C-reactive Protein. In a first incubation step, the CRP in the samples is bound to the coated polyclonal rabbit antibodies (in excess). To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labelled antibody (polyclonal, rabbit-anti- CRP) is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine. An acidic stopping solution is then added. The colour converts to yellow. The intensity of the yellow colour is directly proportional to the concentration of CRP in the sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. CRP, present in the patient samples, is determined directly from this curve. The combination of two specific antibodies in the CRP ELISA drastically reduces the possibility of wrong-negatives results and offers a secure diagnostic system to the user. Range: 1.9-150 ng/ml |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | CRP |
Gene, Accession, CAS # | n/a |
Catalog # | K 9720S |
Price | please inquire |
Order / More Info | CRP ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |