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Product Name | Albumin ELISA Kit |
Description | This Enzyme-Linked Immunosorbent Assay (ELISA) is a two step assay for the ultra sensitive determination of human albumin in stool and urine. A polyclonal rabbit antibody specific for human albumin is immobilized on a microtiter plate and a second anti-albumin antibody is conjugated to peroxidase. In a first incubation step, the albumin in the samples is bound to the immobilized anti-albumin antibodies. A washing step is carried out to remove all unbound substances. In a second incubation step, a peroxidase-labeled anti-albumin antibody is added. After another washing step, to remove all unbound substances, a peroxidase-substrate, tetramethylbenzidine, is added. The enzymatic reaction is terminated by an acidic stop solution, whereby the color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of albumin in the sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. Albumin, present in the patient samples, is determined directly from this curve. Range: 12.5-800 µg/l |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Albumin |
Gene, Accession, CAS # | n/a |
Catalog # | K 6330 |
Price | please inquire |
Order / More Info | Albumin ELISA Kit from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |