ADMA ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | ADMA ELISA |
| Description | This assay is based on the method of competitive enzyme linkedimmunoassays. The sample preparation includes the addition of aderivatization-reagent for ADMA derivatization. Afterwards, the treated samples and the polyclonal ADMA-antiserum are incubated in wells of amicrotiter plate coated with ADMA-derivative (tracer). During the incubationperiod, the target ADMA in the sample competes with the tracer immobilizedon the wall of the microtiter wells for the binding of the polyclonal antibodies.The ADMA in the sample displaces the antibodies out of the binding to thetracer. Therefore, the concentration of the tracer-bound antibody is inverseproportional to the ADMA concentration in the sample.During the second incubation step a peroxidase-conjugated antibody is addedto each microtiter well to detect the anti-ADMA antibodies. After washing awaythe unbound components tetramethylbenzidine (TMB) is added as aperoxidase substrate. Finally, the enzymatic reaction is terminated by an acidicstop solution. The color changes from blue to yellow and the absorbance ismeasured in the photometer at 450 nm. The intensity of the yellow color isinverse proportional to the ADMA concentration in the sample; this means,high ADMA concentration in the sample reduces the concentration of tracerboundantibodies and lowers the photometric signal. A dose response curve ofabsorbance unit (optical density, OD at 450 nm) vs. concentration is generatedusing the values obtained from the standard. ADMA present in the patientsamples is determined directly from this curve. Range: 0.1-2.0 µmol/l |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | ADMA |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7828 |
| Price | please inquire |
| Order / More Info | ADMA ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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