Glutathione HPLC Kit from IMMUNDIAGNOSTIK AG
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| Product Name | Glutathione HPLC Kit |
| Description | The determination of glutathione starts by adding a dilution solution to the sample and dividing it in two aliquots. The measurement of the reduced fraction is performed by the addition of reaction buffer and derivatisation solution. After an incubation of 20 minutes, in which GSH is transformed into a fluorescent product, a precipitation reagent is added to separate higher molecular substances. The measurement of the total glutathione is performed by the addition of the reduction solution, internal standard and derivatisation solution. After that, the sample is handled like the reduced fraction. Depending on the reduction solution all the glutathione (GSH and GSSG) is reduced to GSH. 20 µl of the supernatant are injected into the HPLC system.HPLC analytics Glutathione 18 The separation via HPLC follows an isocratic method at 30 °C using a reversed phase column in two runs. One run lasts 10 minutes. The chromatograms are recorded by a fluorescence detector. The quantification is performed with the delivered EDTA-blood calibrator; the concentration is calculated via integration of the peak area/ height by the external standard method for the reduced fraction and the internal standard method for the total GSH fraction. For the measurement of the reduced fraction it is not possible to use the internal standard because of the production of mixed sulfides. The amount of oxidized glutathione can be calculated by subtraction of: GSHtotal - GSHreduced Note that the obtained difference must be divided by two as oxidized glutathione (GSSG) is composed of two GSH molecules. |
| Size | 100 tests |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Glutathione |
| Gene, Accession, CAS # | n/a |
| Catalog # | KC1800 |
| Price | please inquire |
| Order / More Info | Glutathione HPLC Kit from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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