Histamine ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | Histamine ELISA |
| Description | This assay is based on the method of competitive enzyme linked immunoassays. The sample preparation includes the addition of aderivatization reagent for histamine derivatization. Afterwards, the treatedsamples and a peroxidase-conjugated polyclonal histamine antibody areincubated in wells of a microtiter plate coated with histamine derivative(tracer). During the incubation period, the target histamine in the samplecompetes with the tracer, immobilized on the wall of the microtiter wells, forthe binding of the polyclonal antibodies.After washing away the unbound components tetramethylbenzidine (TMB) isadded as a peroxidase substrate. Finally, the enzymatic reaction is terminatedby an acidic stop solution. The color changes from blue to yellow, and theabsorbance is measured in a photometer at 450 nm. The intensity of the yellowcolor is inverse proportional to the histamine concentration in the sample; thismeans, high histamine concentration in the sample reduces the concentrationof tracer-bound antibody and lowers the photometric signal.A dose response curve of the absorbance unit (optical density, OD at 450 nm)vs. concentration is generated, using the values obtained from the standards.Histamine, present in the patient samples, is determined directly from thiscurve. Range: 0.3-25 ng/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Histamine |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 8212 |
| Price | please inquire |
| Order / More Info | Histamine ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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