Edit |   |
---|---|
Product Name | β-Defensin 2 ELISA |
Description | This ELISA is designed for the quantitative determination of β-defensin 2 in stool. The β-defensin 2 in standards and samples is bound to an available excess of polyclonal antibodies against β-defensin 2, which are immobilised on the surface of the microtiter plate. After a washing step, to remove all interfering substances, the quantification of bound β-defensin 2 is carried out by adding a polyclonal anti β-defensin 2 antibody, which is horseradish peroxidase labeled. After a washing step to remove the unbound components, the peroxidase substrate tetramethylbenzidine is added. Finally, the enzymatic reaction is terminated by an acidic stop solution. The colour changes from blue to yellow and the absorbance is measured in the photometer at 450 nm. The intensity of the yellow colour proportional to the β-defensin 2 concentration in the sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. β-defensin 2, present in the patient samples, is determined directly from this curve. Range: 0.1-3 ng/ml |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Beta Defensin 2 |
Gene, Accession, CAS # | n/a |
Catalog # | K 6500 |
Price | please inquire |
Order / More Info | β-Defensin 2 ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |