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Product Name | anti-htTG sIgA ELISA |
Description | This ELISA is designed for the quantitative determination of anti-htTG sIgA antibodies in faeces. In a first incubation step, the anti-htTG sIgA antibodies in the sample are bound to their antigen (human recombinant transglutaminase), which is immobilised to the surface of the microtiter plates. To remove all unbound foreign substances, a washing step is carried out. In a second incubation step, a peroxidase-labelled sIgA antibody is added. After another washing step, to remove all unbound antibodies, the solid phase is incubated with the substrate, tetramethylbenzidine. An acidic stop solution is then added to stop the reaction. The colour converts from blue to yellow. The intensity of the yellow colour is directly proportional to the amount of bound antibodies and can be determined photometrically at 450 nm. The results are evaluated by comparison with a cut-off value. |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | htTG |
Gene, Accession, CAS # | n/a |
Catalog # | K 9393 |
Price | please inquire |
Order / More Info | anti-htTG sIgA ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |