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Product Name | CRP ELISA Kit |
Description | This Enzyme Immuno Assay is a sandwich assay for the determination of CRP in serum, plasma, urine, dried blood spots and stool samples. The wells of the microtiter plate are coated with polyclonal antibodies directed against C-reactive Protein. In a first incubation step, the CRP in the samples is bound to the coated polyclonal rabbit antibodies (in excess). To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled antibody (polyclonal, rabbit-anti- CRP) is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, Tetramethylbenzidine. An acidic stopping solution is then added. The color converts to yellow. The intensity of the yellow color is directly proportional to the concentration of CRP in the sample. A dose response curve of the absorbance (at 450 nm) unit vs. concentration is generated. CRP, present in the patient samples, is determined directly from this calibration curve. The combination of two specific antibodies in the CRP ELISA drastically reduces the possibility of wrong-negatives results and offers a secure diagnostic system to the user. Range: 1.9-150 ng/ml |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | CRP |
Gene, Accession, CAS # | n/a |
Catalog # | K 9710S |
Price | please inquire |
Order / More Info | CRP ELISA Kit from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |