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Product Name | Coenzyme Q10 HPLC Kit |
Description | The first step in determining coenzyme Q10 includes sample preparation. Therefore the internal standard is added to the calibrator, controls and samples. Higher molecular substances are removed by precipitation and centrifugation. The coenzyme Q10 in the supernatant is then extracted by an organic solvent, which is evaporated afterwards. The residue is resuspended in ethanol p. a. Afterwards, 100 µl of the resuspended solution are injected into the HPLC system. The separation via HPLC follows an isocratic method at 30 °C using a reversed phase column. One run lasts 15 minutes. The chromatograms are recorded by a UV detector. The quantification is performed with the delivered calibrator; the concentration is calculated via integration of the peak areas/heights by the internal standard method. |
Size | 100 tests |
Concentration | n/a |
Applications | n/a |
Other Names | Coenzyme Q10 |
Gene, Accession, CAS # | n/a |
Catalog # | KC1700 |
Price | please inquire |
Order / More Info | Coenzyme Q10 HPLC Kit from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |